An indispensable guide to buffers and to understanding the principles behind their use. Helps the user to avoid common errors in preparing buffers and their solutions.
A must for researchers in the biological sciences, this valuable book takes the time to explain something often taken for granted - buffers used in experiments. It answers the common questions such as: which buffer should I choose? What about the temperature effects? What about ionic strength? Why is the buffer with the biggest temperature variation used in PCR? It provides even the most experienced researchers with the means to understand the fundamental principles behind their preparation and use - an indispensable guide essential for everyone using buffers.
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Acids and bases
Theory of buffer action
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acetic acid acid dissociation constant acid or base acid pKa acids and bases Appendix back-corrected Bicine biological buffer calculations buffer capacity buffer components buffer design buffer preparation buffer solution buffer species buffer system calibration carbonate CH3COOH change in pH Chapter charge chloride Chloroacetic acid compounds concentration of buffer conjugate acid conjugate base correct counterions diluted dissolved dpKa/dT equilibrium example Figure Formic acid free acid glass electrode glycine H3O+ Henderson–Hasselbalch equation hydrogen ion hydrogen ion concentration HyperCard ionic strength Lowry–Brönsted Maleic acid metal ions method NaCl NaOH neutral salt Notes pH electrode pH measurement pH meter pH range pH shift pH unit pH values phosphate Phosphoric acid pKa cited pKa values pKa1 pKa2 potassium Prepare the buffer proton pure water set of buffers standard buffer stock buffer stock solution test solution thermodynamic value titration Tris buffer TrisH+ value cited voltage water molecule weak acids