Buffer SolutionsTaylor & Francis, 1. märts 2004 - 96 pages An indispensable guide to buffers and to understanding the principles behind their use. Helps the user to avoid common errors in preparing buffers and their solutions. A must for researchers in the biological sciences, this valuable book takes the time to explain something often taken for granted - buffers used in experiments. It answers the common questions such as: which buffer should I choose? What about the temperature effects? What about ionic strength? Why is the buffer with the biggest temperature variation used in PCR? It provides even the most experienced researchers with the means to understand the fundamental principles behind their preparation and use - an indispensable guide essential for everyone using buffers. |
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able acetic acid acids and bases activity added addition adjust alkali amount basic biological buffer species calculate calibrate carbonate Chapter charge complete compounds conjugate acid conjugate base consider constant contains correct cover defined definition dependent described detailed diluted discussed dissociation dissolved dpK/dT effects equal equation equilibrium example expected experiments Figure final functional give glass electrode hydrogen ion hydrogen ion concentration increase influence ionic strength ionizable less literature lower mean measure method negative neutral Notes pH changes pH meter pH range pH values phosphate pKa values possible practical prepare present problem proton pure water range reaction reading recipes reference relatively resist salt shift simple sodium solution sometimes standard strong Table temperature term theory thermodynamic titration Tris understanding units usually voltage volume